Trivalent Inactivated Influenza Vaccine (Fluzone®)

To evaluate the generalized utility of the LTE module for supporting in vitro B cell responses, we performed similar assays as described above, but used influenza as the antigen. With this approach, DCs were cultured overnight with no antigen, a trivalent influenza vaccine (Fluzone®) or heat-killed (HK) influenza virus strain A/New Caledonia/20/99 (A/NC).

After 7 days, the total number of influenza-specific B cells was calculated by ELISPOT assay using plate-bound HK A/NC as the target antigen. The LTE module demonstrates a clear advantage of over unfractionated PBMC in generating influenza-specific antibody responses. The results below, which are representative of 3 experiments, demonstrate that the frequency of antigen-specific B cells in LTE cocultures was nearly 10-fold higher than in unfractionated PBMC, which further substantiates our hypothesis that MIMIC® cultures provide a superior assay method for the in vitro detection of human B cell responses.